r2 9a5  (Bio X Cell)

Journal: The EMBO Journal

Article Title: T-cell-derived IFN-γ suppresses T follicular helper cell differentiation and antibody responses

doi: 10.1038/s44318-025-00414-3

Figure Lengend Snippet: Reagents and tools table

Article Snippet: InVivoMab α-IL-12 blocking antibody , BioXcell , Clone R2-9A5 #BE0233.

Techniques: Transgenic Assay, Mouse Assay, Recombinant, Blocking Assay, Control, Staining, Sequencing, Expressing, Derivative Assay, Lysis, Software, Cell Isolation, Microscopy, Real-time Polymerase Chain Reaction

Journal: Cell reports

Article Title: TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4 + T Cells to T reg

doi: 10.1016/j.celrep.2020.01.040

Figure Lengend Snippet: (A and B) Groups of C57BL/6 and TLR-deficient mice infected with LD or HD Lm Δ actA -Ova. (A) Absolute number of Ova-specific CD8 + T cells at day 7 post-infection with HD Lm Δ actA -Ova compared among splenic isolates of Tlr2 −/− , Tlr4 −/− , Tlr7 −/− , and Tlr9 M7Btlr mice (n = 6–10 per group). (B) Absolute number of Ova-specific CD8 + T cells at day 7 in the spleens of Tlr9 M7Btlr mice ± CD4 + T cell depletion (n = 6–8 per group). (C and D) Groups of C57BL/6 and Tlr9 M7Btlr mice systemically infected with HD Lm Δ actA -Ova. (C) Fold change in serum cytokines and chemokines from 0–6 h post-infection. (D) Selected serum cytokine/chemokine concentration at 6 h post-infection displaying significance in (C) (n = 6 per group). (E) Foxp3 EGFP mice infected with HD Lm Δ actA -Ova for 24 h. Ingenuity Pathway Analysis (IPA) upstream regulator prediction of differential gene expression profiles comparing total FoxP3-EGFP + T reg sorted from naive versus infected mice (top 50 significant hits; p = gene network overlap) (n = 4 per group). (F) Kinetic display of IFN-a2/4, IFN-b, IFN-g, and IL-12 (p70) serum concentration in C57BL/6 and Tlr9 M7Btlr mice after exposure to HD Lm D actA -Ova (n = 6 per group).

Article Snippet: ant-IL-12 (p70) (R2-9A5) , BioXCell , Cat#BE0233.

Techniques: Infection, Concentration Assay, Gene Expression

Journal: Cell reports

Article Title: TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4 + T Cells to T reg

doi: 10.1016/j.celrep.2020.01.040

Figure Lengend Snippet: (A) C57BL/6 mice administered 50 μg CpG-A, CpG-B, and CpG control ODNs at days −1 and 0. Frequency of splenic CD25 + FoxP3 + CD4 + T reg and change in FoxP3 + :FoxP3 − CD4 + T cell ratio relative to CpG controls (D FoxP3 + :FoxP3 − ) at day 1 (n = 4 per group). (B) C57BL/6 and Foxp3 DTR-EGFP mice administered CpG ODNs as in (A) and infected with LD Lm Δ actA -Ova at day 0 and co-delivered a complete DT regimen. Absolute number of splenic Ova-specific CD8 + T cells at day 7 post-infection (n = 6 per group). (C) Splenic Ova-specific CD8 + T cell response observed in CpG-treated or -infected C57BL/6 as in (B), with blockade of IL-12 (p70) (n = 4 per group). (D) Quantification of mtDNA ( 12 s and Trlev ), nDNA ( Neb and Becn1 ), and Lm DNA ( Ova , mpl , and plcB ) in cell- and RNA-free serum samples after HD Lm Δ actA - Ova infection of C57BL/6 mice (n = 6 per group). (E) Serum ALT concentration within LD versus HD Lm Δ actA -Ova-infected C57BL/6 mice (n = 6 per group). (F) Effect of neutrophil depletion before HD Lm Δ actA -Ova infection of C57BL/6 mice on mtDNA ( 12 s and Trlev ) in cell- and RNA-free serum samples isolated at 4 h post-infection (n = 6 per group). (G) Absolute number of splenic Ova-specific CD8 + T cells at day 7 post-infection in C57BL/6 and Foxp3 DTR-EGFP mice administered a complete DT regimen, systemic HD Lm Δ actA -Ova, and selective DNase I delivery (n = 4 per group). (H) Survival of untreated versus DNase I-treated C57BL/6 mice infected with WT Lm Ova (n = 10–16 per group). (I and J) In vitro co-expression of FoxP3 and T-bet in conventional CD4 + EGFP − T cells isolated from Foxp3 EGFP or Il12rb2 −/− Foxp3 EGFP mice cultured in the presence or absence of CD3/CD28-mediated activation, rmTGF-b1, and rmIL-12 (p70) (n = 4 per group).

Article Snippet: ant-IL-12 (p70) (R2-9A5) , BioXCell , Cat#BE0233.

Techniques: Control, Infection, Concentration Assay, Isolation, In Vitro, Expressing, Cell Culture, Activation Assay

Journal: Cell reports

Article Title: TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4 + T Cells to T reg

doi: 10.1016/j.celrep.2020.01.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ant-IL-12 (p70) (R2-9A5) , BioXCell , Cat#BE0233.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Gene Expression, Software, Luminex